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Kishor Kumar, V.
- Antihyperglycemic, Antihyperlipidemic and Antioxidant Activities of Ethanolic Extract of Helleborus niger Linn Roots in Streptozotocin - Nicotinamide Induced Diabetic Rats
Authors
1 Department of Phytopharmacy and Phytomedicine, J. K. K. Munirajah Medical Research Foundation's Annai J. K. K. Sampoorani Ammal College of Pharmacy, B. Komarapalayam – 638 183, Namakkal District, Tamil Nadu, IN
2 Department of Pharmaceutical Chemistry, Ultra College of Pharmacy, Madurai – 625 020, Tamil Nadu, IN
Source
Journal of Pharmaceutical Research, Vol 13, No 3 (2014), Pagination: 92-101Abstract
Helleborus niger L (HN) is widely used in the treatment of diabetes mellitus in the Indian traditional system of medicine. Therefore, the present study was done to evaluate the antihyperglycemic, antihyperlipidemic and antioxidant activities of ethanol extract of HN ischolar_main (EHN) in streptozotocin (STZ) - nicotinamide (NC) induced diabetic rats. in vitro α-amylase inhibition assay, normoglycemic study and oral glucose tolerance test were carried out in different ischolar_main extracts of HN. Antihyperglycemic effect was assessed in EHN using diabetic rats, which was identified as the most effective extract by initial screening. EHN (50, 100 and 200 mg/kg) and glibenclamide (5 mg/kg) orally administered daily for 28 days and the animal was observed in next 14 days. Fasting blood glucose (FBG) and body weight was determined weekly basis up to 42 days. On the 42nd day, various biochemical parameters were estimated. The three doses of EHN shows significantly decrease (p<0.01) in blood glucose levels. The effect was more pronounced in 200mg/kg (71.53%) than 100mg/kg (67.46%) and 50mg/kg (66.63%). In addition, decreased HbA1C and improved Hb level were 1 evidenced clearly in diabetic rats. Simultaneously, improvements in serum lipid profile, serum liver profile in diabetic rats were also evidenced clearly. Moreover, body weight and protein levels were increased in diabetic rats. On the other hand antioxidant activity was restored in diabetic rats. Increased glycogen content, glucokinase and decreased glucose - 6 phosphatase, fructose 1, 6 - biphosphatase effects in liver tissues were observed. EHN preserved islet architecture and prevented hypertrophy of β-cells. The EHN is capable of managing hyperglycemia and complications of diabetes in STZ-NC induced diabetic rats. Hence this plant may be considered as one of the potential sources for the isolation of new oral antihyperglycemic agent(s).Keywords
Antihyperglycemic, Antihyperlipidemic, Antioxidant, Helleborus Niger, Nicotinamide, Streptozotocin.- In-vitro Anti Oxidant and Antimicrobial Activities of Ethyl Acetate Extract of Evodia lunu-Ankenda (Gaertn) Merr. Bark
Authors
1 Department of Pharmaceutical Chemistry, JKKMMRF College of Pharmacy, B. Komarapalayam, Tamil Nadu, IN
Source
Research Journal of Pharmacognosy and Phytochemistry, Vol 1, No 3 (2009), Pagination: 201-203Abstract
In this paper, in vitro antioxidant and antimicrobial activities of ethyl acetate extract of Evodia lunu-ankenda (Gaertn) Merr. bark was determined by four methods Diphenylpicrylhydrazyl (DPPH), Nitric Oxide, Super oxide disumatase, Hydrogen peroxide (H2O2) and by agar disc diffusion methods. The crude ethyl acetate extract of Evodia lunuankenda bark inhibited the growth of both gram positive bacteria (Bacillus substilis, Staphylococcus aureus and micrococcus luters) and gram negative bacteria (Escharichia coil, Pseudomonas aeruglinosa and Salmonella typhlmurium) and also the crude ethyl acetate extract of Evodia lunuankenda bark inhibited the growth of fungi (candida albicans and Aspergillus niger). The ethyl acetate extract of Evodia lunuankenda bark showed dose dependent increase in reducing anti oxidant power that was comparable to standards. Antibacterial activity, highest concentration of ethyl acetate extract (5mg/ml) having good activity against gram positive and gram negative organisms as compared with the reference Ciprofloxacin. Antifungal activity, highest concentration of extract (5mg/ml) having good activity as compared with the reference Amphotericin-B. It can be concluded that the plant posses potent antioxidant and antimicrobial activity, responsible of the secondary metabolites like flavonoids, saponins, are likely observed for the plants.
Keywords
Antioxidant Activity, Antimicrobial Activity, Agar Disc Diffusion Method, Evodia lunu-Ankenda (Gaertn) Merr. Bark.- RP-HPLC Method for Simultaneous Determination of Pioglitazone and Telmisartan in Tablet Dosage Form
Authors
1 Dept. of Pharmaceutical Chemistry, JKKMMRF College of Pharmacy, Komara Palayam-638183, Tamil Nadu, IN
2 Department of Pharmaceutical Chemistry, Ultra College of Pharmacy, Madurai-625020, Tamil Nadu, IN
Source
Asian Journal of Research in Chemistry, Vol 3, No 3 (2010), Pagination: 585-587Abstract
A Simple and precise reverse phase high performance liquid chromatographic (RP-HPLC) method was developed and validated for the quantitative determination of Diacerein in bulk and its pharmaceutical formulations. Chromatographic separation was performed by using column XDB C8 (4.6×150 mm, 5 μm) with a mobile phase comprising of a mixture of phosphate buffer and Acetonitrile (50:50,v/v) and pH adjusted to 3 with orthophosphoric acid, at a flow rate of 0.8 ml/min with detection at 254 nm. Separation was completed in less than 10 min. As per ICH guidelines the method was validated for linearity, accuracy, precision, limit of quantitation, limit of detection, ruggedness and robustness. Linearity of Diacerein was found to be in the range of 10-100 μg/ml and correlation coefficient was found to be 0.999. The retention time of Diacerein was 3.3 min;the separation was performed at an ambient temperature. The limit of Detection and limit of quantitation for Diacerein was found to be 3.6 and 9.95 (S/N ratio). There was no significant difference in the intraday and inter day analysis of Diacerein determined for five different concentrations using this method. The RSD value 0.3% indicates a high precision of the analytical method.Keywords
The Proposed Method was Simple, Sensitive, Precise, Accurate and Useful for Routine Quality Control Analysis.- New Spectrophotometric Method Applied to the Simultaneous Determination of Metoprolol Succinate and Hydrochlorthiazide
Authors
1 JKKMMRF College of Pharmacy, B. Komarapalayam, IN
2 ROVER College of Pharmacy, Perambalur, IN
3 ULTRA College of Pharmacy, Madurai, IN